Enzyme Lab Report

Group 1-1 Names

JT Wellman,  Gabriella Lasserre, Katarina Myers,  Lupe Castillo

Introduction:

In this lab, we decided to investigate the effect of environment factors on the enzyme hydrogen peroxidase. To do that we decided to test the substrate concentration and how it affects the enzyme’s productivity. For this we used ripgut brome grass(bromus diandrus), substrate and water.

Materials:

• 5 to 10 grams of freshly picked ripgut brome grass (Bromus diandrus) blades (about 1 handful)
• Digital balance (scale)
• Mortar and pestle
• 3 100-liter glass or plastic beakers
• 1 mL or 5 mL syringe
• Hydrogen peroxide
• Glass test tubes
• Test tube rack or holder
• Safety glasses

Procedure:

1) Gather materials

2) Put on safety glasses

3) Set up your lab with materials.

4) Weigh the brome grass to make sure it is between 5 to 10 grams

5) Grind the grass with the mortar and pestle

6) Pour the solution into 5 test tubes

7)Then we pour different amounts of the hydrogen peroxide ( 10%, 20%, 40%, 50%, 80% ) in the test tubes

8) Measure and record the height of the reaction

9) Write data results and conclusion

Hypothesis

If the substrate concentration increases then the rate of enzymatic reactions will increase to a certain point then steady out.

Independent Variable: substrate concentration

Dependent Variable: peroxidase

Controlled Variables: temperature, pH, enzyme concentration

We chose this because when substrate concentration goes higher, it increases enzyme productivity because there are more substrates to work on.

Summary:

We are going to test how enzymes are affected when the controlled variable of substrate is added to the equation. We will be testing this by running 5 different experiments and placing varying amounts of hydrogen peroxide in each to see the result and record it.

Data and Results:

At 80% of substrate concentration with 5 ml of enzymes solution we found that we had 6 ml of peroxidase in 10 minutes. At 50% of substrate concentration with 5 ml of enzymes solution we found that we had 9 ml of peroxidase in 10 minutes. At 40% of substrate concentration with 5 ml of enzymes solution we found that we had 10 ml of peroxidase. At 20% substrate concentration with 5 ml of enzymes solution we found that we had 8 ml of peroxidase in 10 minutes. At 10% of substrate concentration with 5 ml of enzymes solution we found that we had  3.5 ml of peroxidase in 10 minutes.

Conclusions:

Many steps were taken in order for us to achieve our results for this lab. By the end of the lab, we analyzed the results and concluded that increasing substrate concentration increases enzyme productivity. However, at a certain point, the productivity decreases. As seen in our data, we got the most results of ml of peroxidase at 50% substrate concentration. 50% substrate concentration gave us 9ml of peroxidase.